Abstract:
Lice living on farm animals and human body as ecto-parasites are
considered to be annoying problem. Some of the debusing formulae are found
to be ineffective due to emergence of resistant starins and other health hazards.
Therefore, it has become a long felt need to find out an amicable solution to
this problem. Annona muricata seeds have been used since time immemorial to
control human head lice {Pediculus humanus capitis). Main objectives of this
study was to check the effectiveness of seed oil of Annoha against human head
louse by conducting bio-assay, preparation of a suitable formula as a shampoo
incorporating above extract and check any adverse effect of this product on
mammals.A formulation o f shampoo was prepared using the crude oil of
Annona muricata. De-ionized water, surfactant (Sodium Laureth Ether
Sulfate), sodium chloride, citric acid, a natural colourings and a fragrance were
used to prepare the shampoo. The licicidal properties of the shampoo
formulation were tested in vitro using chicken louse and human head louse in
the laboratory. Shampoo did not show any toxic or adverse effect on gunea
pigs when applied topically. Further test was carried out on louse infected child
who volunteered. Test showed that LT 50 values (30 minutes) were 12, 14,and
25 for 10%, 5%, 2% and 1% respectively for human head lice. After 30
minutes 100% mortality was observed in 10%, 5% and 2% shampoo. Treated
persons recovered from head lice infestation without any abnormal symptoms
due to treatment. The shampoo prepared with 5% Annona seed oil could be
effectively used as a safe botanical licicide for control human head lice.
