Abstract:
Background: Since synthetic medications used in hyperlipidemia cause undesirable side effects
upon long term administration, prompting interest in natural remedies for their non-toxic
properties. Thus, Kalanchoe pinnata and Atalantia ceylanica plants which were scientifically
proven with serum cholesterol reducing activity were considered in this study.
Objectives: To evaluate the lipase inhibitory activity and toxicity profile of the aqueous leaf
extracts of K. pinnata and A. ceylanica, to formulate a polyherbal syrup and evaluate
physicochemical properties and stability
Methods: The aqueous macerated products of each fresh leaves were freeze-dried and test
samples of (15000, 12000, 11000, 10000, 5000, 2500 ppm) were prepared to evaluate in vitro
lipase inhibitory and brine shrimp lethality assays. The lipase inhibitory assay was triplicated
using Orlistat as the positive control. The 1 mg/mL of 1.5 mL porcine pancreatic lipase enzyme
and 1.5 mL of each test sample were mixed and incubated at 30℃ for 1hour. Then 3 µL of
10mM P-nitro phenyl butyrate substrate was added, re-incubated for 5 minutes at 30℃ and
absorption was measured at 405 nm. Five different syrup bases were prepared using distilled
water, saccharin, sodium benzoate, citric acid and subjected to evaluate stability and
physicochemical properties at room temperature. Three different extract ratios of K. pinnata and
A. ceylanica were incorporated to the stable base and three polyherbal syrups were prepared and
evaluated lipase inhibitory activity, color, odor, appearance and pH along with long-term and
short-term stability.
Results: The 15000 ppm concentration showed the highest lipase inhibition for the K. pinnata
and A. ceylanica (36.68%±0.002 and 76.02%±0.004, respectively) compared to the positive
control (IC50 1224.75 ppm). The polyherbal syrup with extraction ratio of 1.5: 1.1 showed the
highest enzyme inhibition (63.06%±0.008) with non-toxic nature. This formulation was stable at
room temperature to date with odorless, clear pale green appearance and the pH was at the
acceptance range of pH 4.0-6.9.
Conclusion: The stable polyherbal syrup which showed 63% of lipase inhibition with no toxic
effects can be considered for further development to treat hyperlipidemia.
