Abstract:
Salmonella enterica, Vibrio parahaemolyticus, Escherichia coli and Listeria monocytogenes are human
pathogenic bacteria which could be contaminated with many types of food, especially meat products.
Testing of food for those pathogens has become routine practice all over the world. However, since the
available traditional detection methods are time consuming and labor intensive, there is a need for
rapid, sensitive, specific and cost effective detection technique. In this study, a rapid and sensitive single
tube Multiplex PCR method for simultaneous detection of those four bacterial pathogens by amplifica tion of target genes gyrB of V. parahaemolyticus, invA of S. enterica, eaeA of E.coli and hly of L. monocy togene was established. Concentration of bacterial cells from food samples for DNA extraction is an other problem in testing of food pathogens by PCR techniques. Metal hydroxide immobilization was
applied prior to DNA extraction and found to be effective step to overcome this problem.
