DNA typing of dessert banana cultivar „kolikuttu‟ („silk‟) accessions by microsatellite markers

Abstract

Fourteen Kolikuttu accessions showing morphological variation and Fusarium wilt tolerance were col lected. Extracted DNA was subjected to PCR using six SSR primers. PCR products were separated by PAGE and bands were visualized, scored and statistically analyzed using POPGENE 1.32 version to obtain the dendrogram by genetic distance matrix. Out of 19 SSR alleles amplified, 12 were polymor phic resulting in 63.16% loci polymorphism. Based on the dendrogram, three different „Kolikuttu’ ac cessions known as “Athdath Puwalu”, the accession collected from Telijjawila, and accession collected from Angunakolapelassa were clustered separately. These three accessions could be considered as dif ferent genotypes.