Abstract:
An efficient in vitro propagation culture system was developed for purple coneflower. In the system,
adventitious buds were first regenerated from leaf, petiole and root explants of aseptic seedlings on MS
medium with 0.3 mg/l BA and 0.01 mg/l NAA, and then these adventitious buds were rooted and grown
into more mature plantlets on MS media with 0.01 mg/l NAA for providing more explant materials for
a subsequent adventitious bud regeneration culture. It was found that explants taken from plantlets of
different maturity had different adventitious bud regeneration ability, with those from two and a half
months old plantlets having the highest regeneration ability, and with root explants having the highest
number of adventitious buds regenerated. Through this culture system, it was possible to obtain at least
one million plantlets ready for transplantation within one year.
