Abstract:
This study was conducted to investigate the effects of explant source and hormone concentrations on the callogenesis, calli growth, plantlet regeneration and rooting of a local tomato variety (Lycopersicon esculentum mill. variety ‘Thilina’). Different combinations of Benzyl Adenine (BAP) and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) were used with hypocotyl, leaf and root explants in a completely randomized design with five replicates to evaluate the success in plantlet regeneration. Regenerated healthy shoots sub cultured in to MS medium with various
concentrations of Indole-3-butric acid (IBA) for rooting.After one month the weight of fresh callus, number of regenerated shoots and roots were evaluated. Anova (DMRT) test shows there were significant effects at p<0.05 level. Combination of BAP (0.1mgl-1) with 2.4-D (2.0mgl-1) and hypocotyl explant produced the best quality fresh callus in highest weight. The best hormonal combination for shoot regeneration (4 shoots/explant) was 0.1mgl-1 NAA and 0.5mgl-1 Kinetin from callus. Maximum direct regeneration was observed on MS medium containing 0.5mgl-1 Ki netin, 2.0mgl-1 BAP, 0.1mgl-11-Naphthaleneacetic acid (NAA) and 100mgl-1 my-inositol within 15-20 days (4 shoots/explant). Leaf bud revealed to be better explants for direct regeneration. Highest root number per plantlet was observed with 2.0mgl-1
IBA.
