Abstract:
Banana (Musa spp.) is one of the most important fruit crops globally, and in-vitro propagation plays a crucial role
in large-scale multiplication, particularly for cultivars like sour bananas (Mysore AAB). In tissue culture, sucrose
is the primary carbon source, typically used in the form of commercial white sugar. However, this study
investigates the potential of sugarcane molasses as a cost-effective and sustainable alternative to commercial white
sugar in Murashige and Skoog (MS) medium for in-vitro sour banana propagation. The experiments were
conducted using MS medium supplemented with varying concentrations of sugarcane molasses (10 g/L, 20 g/L, 30
g/L, and 40 g/L) compared to the standard 30 g/L of commercial white sugar. Growth parameters such as root
initiation, root number, root length, plant height, root fresh and dry weight, and total plant fresh and dry weight
were evaluated over six weeks. The MS medium with sugar (T1) showed the best results, significantly
outperforming all other treatments (p <0.05), with the highest plant height (53.36 mm), root number (5.41), root
length (7.68 cm), and plant dry weight (0.09 g). Sugarcane molasses in low concentration with MS media (T2, 10 g/
L) exhibited promising performance, it significantly lower than T1 with plant height (34.4 mm), root number
(4.4), and shoot dry weight (0.05 g). However, higher concentrations of molasses resulted in media browning and
reduced root growth, likely due to excessive impurities and phenolic compound accumulation. This study
demonstrates that sugarcane molasses in low concentrations with further optimization could be a viable, cost
effective, and sustainable alternative carbon source for commercial micro propagation of banana. Therefore,
further research is recommended to refine molasses treatment methods to minimize impurities and optimize
plantlet development.
